A specific, accurate, precise and reproducible method has been developed and validated for the simultaneous estimation of amoxycillin trihydrate and tinidazole in combined dosage form by UV Spectrophotometer by applying Simultaneous Equation method. For development of method wavelengths selected were 229 nm and 310.40 nm for estimation of amoxycillin trihydrate (AMX) and tinidazole (TIN) respectively. The two drugs obeyed Beer-Lambert’s law over the concentration range of 8-18 μg/ml for amoxycillin trihydrate and 6-21 μg/ml for tinidazole. Validation of the proposed method was carried out for its specificity, linearity, range, accuracy, precision, Limit of detection, Limit of quantitation according to ICH guidelines. Recovery was found in the range of 99.41 to 100.47% for tinidazole and amoxycillin trihydrate in the formulation. The % assay of tinidazole and amoxycillin trihydrate was found to be in the range 99.54–94.90% by the proposed method. The results of analysis have been validated statistically and recovery studies confirmed the accuracy and reproducibility of the proposed method which was carried as per ICH guidelines. The proposed method was successfully applied in routine work for the determination of amoxycillin trihydrate and tinidazole in combined tablet dosage form.
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